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                 )          %                               ,      
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  
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  	 	        " = $  ( &   3 "  ¨ U  ,   )  f   zÅ  |K  |k  |Ã  ‚'  ˆ1  �‰Ÿ	ùUŒÙñ
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Ud–d5––bé	Í
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mÂòGÊN»
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<+ó"ÌÅáô¨sÄóŸi3
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õ
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¤­ea 5üg·"CZ·ù	ÒËÐÌ=C8âª¸ã³£»ä²=z	 lOF^¾£Z·ù	ÒÐ=y	lY_qÕ‹fš ûYnW<—Ï¯è2) Ò"Cvòz GZ5|lŠ œ|ÑmÎÚ �#	hÂ&yÛ«ìRJÝôQ�©ÌKê6@êì	=
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<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="6:50:25">2019.6.2</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite><BindingSite simplified="1" location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="91"><Component hybridizedRange="2077-2326" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite></Primer><Primer recentID="22" name="M13R" sequence="CAGGAAACAGCTATGAC"><BindingSite location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="17" name="V1_5" sequence="GGACTTTCCAAAATGTCG"><BindingSite location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite><BindingSite simplified="1" location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite></Primer><Primer recentID="16" name="HH-pBRori-cexuR" sequence="TCGGGTTTCGCCACCTCTG"><BindingSite location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite></Primer><Primer recentID="12" name="PQE-F" sequence="TGAGCGGATAACAATTTCAC"><BindingSite location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite><BindingSite simplified="1" location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite></Primer><Primer recentID="11" name="PCMV-R" sequence="TCCAAACTCATCAATGTATC"><BindingSite location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite><BindingSite simplified="1" location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite></Primer><Primer recentID="10" name="PIRES-R" sequence="CACTGCATTCTAGTTGTGGT"><BindingSite location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite><BindingSite simplified="1" location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite></Primer><Primer recentID="9" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC"><BindingSite location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="5" name="EBV-Reverse" sequence="CAGCTTATAATGGTTACAAAT"><BindingSite location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite><BindingSite simplified="1" location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite></Primer><Primer recentID="3" name="CMV-Forward" sequence="CGCAAATGGGCGGTAGGCGTG"><BindingSite location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite><BindingSite simplified="1" location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite></Primer><Primer recentID="2" name="PEGFP-N-5" sequence="TGGGAGGTCTATATAAGCAGAG"><BindingSite location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite><BindingSite simplified="1" location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite></Primer><Primer recentID="0" name="M13R(-48)" sequence="AGCGGATAACAATTTCACACAGGA"><BindingSite location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite><BindingSite simplified="1" location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="14:0:49">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  ö<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3031-3047" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3007-3023" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3100-3121" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3055-3061" color="#ffffff" type="standard"/><Segment range="3062-3079" color="#ffffff" type="standard"/><Segment name="-35" range="3080-3085" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5026-5130" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2837-2958" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1920-2055" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1028-1252" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1740-2069" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1298-1726" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3409-3994" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4956" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4165-4956" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4957-5025" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2078-2677" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ÷<?xml version="1.0"?><Primers recycledIDs="21,19,1,4,14,18,7,13,20,6" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="Primer 1" sequence="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite><BindingSite simplified="1" location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite></Primer><Primer recentID="22" name="M13R" sequence="CAGGAAACAGCTATGAC"><BindingSite location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="17" name="V1_5" sequence="GGACTTTCCAAAATGTCG"><BindingSite location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite><BindingSite simplified="1" location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite></Primer><Primer recentID="16" name="HH-pBRori-cexuR" sequence="TCGGGTTTCGCCACCTCTG"><BindingSite location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component 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bases="TCCAAACTCATCAATGTATC"/></BindingSite></Primer><Primer recentID="10" name="PIRES-R" sequence="CACTGCATTCTAGTTGTGGT"><BindingSite location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite><BindingSite simplified="1" location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite></Primer><Primer recentID="9" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC"><BindingSite location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" 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  c<Notes>
<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="14:0:38">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="13:37:25">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:49:10">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:49:5">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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  f<Notes>
<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:44:14">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<LastModified UTC="3:40:28">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<LastModified UTC="3:36:6">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:30:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:28:32">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:26:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:24:48">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<LastModified UTC="3:22:29">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>5491a808-6553-4aaa-b42b-d41df4abc516</UUID>
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<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-CHAT(human)-3Ã—FLAG-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="19:5:47">2025.9.20</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             ë<StrandColors><TopStrand><ColorRange range="0..929" colors="black"/><ColorRange range="930..1256" colors="light blue"/><ColorRange range="1257..2315" colors="black"/><ColorRange range="2316..2316" colors="magenta"/><ColorRange range="2317..2575" colors="black"/><ColorRange range="2576..2576" colors="magenta"/><ColorRange range="2577..7428" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..929" colors="black"/><ColorRange range="930..1256" colors="light blue"/><ColorRange range="1257..2315" colors="black"/><ColorRange range="2316..2316" colors="magenta"/><ColorRange range="2317..2575" colors="black"/><ColorRange range="2576..2576" colors="magenta"/><ColorRange range="2577..7428" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
